POLYPEPTIDE HAVING FIBRIN-BONDING ACTIVITY

• Main Authors: KIMIZUKA FUSAO, ICHIMURA NOBUKO, KATOU IKUNOYUKI, OKAZAWA KAZUHIDE, NODA AKIHIRO
• Format: Patent
• Language: English
• Published: 27.05.1991
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; SUGARS; VINEGAR; WINE

NEW MATERIAL:The peptide of formula I (W is Met; X is Ala; Y is 11 amino acid polypeptide residue of human fibronectin having the sequence of formula II; Z is 132 amino acid polypeptide residue of fibrin-bonded domain at the C- terminal side of human fibronectin having the sequence of formula III; (l), (m) and (n) are 0 or 1). USE:A blood coagulation inhibitor. A material for drug-delivery system. PREPARATION:The objective polypeptide of formula I can be produced e.g. by treating a cDNA fragment coding the 143 amino acid sequence at the C- terminal side of human fibronectin (FN) with a restriction enzyme, subjecting the treated product to agarose gel electrophoresis, recovering the fragments of 0.96kb and 405kb, bonding the fragments to an adaptor, conjugating to a vector, cloning the product, transducing into E.coli to effect transformation and culturing the resultant transformant.