RAPID DETECTION OF FINE AMOUNT OF ANAEROBIC BACTERIUM

• Main Author: TOTO MASANORI
• Format: Patent
• Language: English
• Published: 27.05.1991
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PURPOSE:To remarkably shorten a detection period by capturing anaerobic bacteria in a specimen solution with a filter, subjecting the captured bacteria to a specific treatment and subsequently measuring adenosine-3-phosphoric acid in the bacteria remaining on the filter. CONSTITUTION:A specimen solution containing anaerobic bacteria is filtered through a filter having pore sizes of <=0.45 mum to capture the bacteria on the filter. The captured bacteria are inoculated on an agar-containing medium and cultured under an anaerobic atmosphere for 12-48 hours. The filter after the culture is washed with a washing liquid to remove the medium components adhered to the filter, followed by measuring the amount of adenosine-3- phosphoric acid in the bacteria remained on the filter.