RAPID DETECTION OF FINE AMOUNT OF ANAEROBIC BACTERIUM

PURPOSE:To remarkably shorten a detection period by capturing anaerobic bacteria in a specimen solution with a filter, subjecting the captured bacteria to a specific treatment and subsequently measuring adenosine-3-phosphoric acid in the bacteria remaining on the filter. CONSTITUTION:A specimen solu...

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Bibliographic Details
Main Author TOTO MASANORI
Format Patent
LanguageEnglish
Published 27.05.1991
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To remarkably shorten a detection period by capturing anaerobic bacteria in a specimen solution with a filter, subjecting the captured bacteria to a specific treatment and subsequently measuring adenosine-3-phosphoric acid in the bacteria remaining on the filter. CONSTITUTION:A specimen solution containing anaerobic bacteria is filtered through a filter having pore sizes of <=0.45 mum to capture the bacteria on the filter. The captured bacteria are inoculated on an agar-containing medium and cultured under an anaerobic atmosphere for 12-48 hours. The filter after the culture is washed with a washing liquid to remove the medium components adhered to the filter, followed by measuring the amount of adenosine-3- phosphoric acid in the bacteria remained on the filter.
Bibliography:Application Number: JP19890260236