APPARATUS FOR AUTOMATIC SIMULTANEOUS DETERMINATION OF CELL CONCENTRATION AND CELL ACTIVITY

• Main Authors: GONDA KINJI, HAKETA YASUSHI, MOTOHASHI RYOICHI, KAJIWARA KAZUTO
• Format: Patent
• Language: English
• Published: 27.05.1991
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

PURPOSE:To determine the cell concentration and cell activity in a cell suspension liquid at the same time by placing a chemiluminescence detector in a detection channel and an absorbance detector in a sample supplying channel, etc., mixing a sample supplied through a sample channel and a reagent supplied through a reagent channel in a mixer and transferring the mixture through the detection channel. CONSTITUTION:A sample liquid S containing suspended cells in injected into a carrier liquid C1 and an ATP extraction liquid R is injected into the carrier liquid before a mixer 11. A luminescent reagent E containing luciferin and luciferase is injected into a carrier liquid C2 and the sample liquid is mixed to the reagent liquid with a mixer 12. A sample channel 2 is joined to a reagent channel 4 and the mixture is passed through the mixer 13 to a chemiluminescence detector 6 and an absorbance detector 7 to determine the ATP concentration and turbidity. The ATP concentration in the cell and the concentration outside of the cell can be separately determined by injecting the ATP extraction liquid R and without using the injection of the liquid.