PRODUCTION OF PROTEIN WITH YEAST AS HOST

PURPOSE:To obtain the title protein of higher organism origin markedly improved in its secretory productivity by feeding a carbon source and/or nitrogen source at amount(s)s more than the minimum amount required for the proliferation of yeast in producing a foreign protein using the transformed yeas...

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Bibliographic Details
Main Authors CHIGAMI YOSHIFUMI, FURUMIYA KOJI
Format Patent
LanguageEnglish
Published 17.09.1990
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To obtain the title protein of higher organism origin markedly improved in its secretory productivity by feeding a carbon source and/or nitrogen source at amount(s)s more than the minimum amount required for the proliferation of yeast in producing a foreign protein using the transformed yeast. CONSTITUTION:When a foreign protein is to be produced using yeast transformed by secretory manifestation vector containing promoter and alien gene, a carbon source and/or nitrogen source is (are) fed to a culture medium, at amount(s) more than the minimum amount required for the proliferation of the transformed yeast. For example, as the carbon source, 80-150g/l of glucose or succharose, and as the nitrogen source, 10-30g/l of ammonium sulfate are fed, respectively. And, as inorganic nutritive salts, for example, 1-3g/l of dipotassium phosphate, 1-3g/l of magnesium sulfate heptahydrate, 0.5-2g/l of potassium chloride, 10-50mg/l of ferrous sulfate heptahydrate, etc., are fed, respectively.
Bibliography:Application Number: JP19890055169