PRODUCTION OF HIGH-PURITY BOVINE IMMUNOGLOBULIN

PURPOSE:To produce high-purity bovine immunoglobulin with simple operation by desalting whey or concentrated whey protein and contacting with a cation exchange material within a specific pH range. CONSTITUTION:Whey or a concentrated whey protein having a protein concentration of >=0.5wt.% is desa...

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Bibliographic Details
Main Authors TOMIMURA TOSHIO, MIZOTA TERUHIKO, TOMITA MAMORU
Format Patent
LanguageEnglish
Published 17.04.1990
Subjects
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:PURPOSE:To produce high-purity bovine immunoglobulin with simple operation by desalting whey or concentrated whey protein and contacting with a cation exchange material within a specific pH range. CONSTITUTION:Whey or a concentrated whey protein having a protein concentration of >=0.5wt.% is desalted to an electrical conductivity of <=2mS/cm. The desalted liquid is adjusted to pH 4.5-5.5 and made to contact with a cation exchange material (preferably alkali metal-type) at 0-40 deg.C to effect the adsorption of the immunoglobulin. The ion exchange material is recovered, washed with water adjusted to pH 4.5-5.5 to completely wash off the unadsorbed whey protein and eluted with a salt solution to obtain a high-purity immunoglobulin containing >=55% of immunoglobulin based on the protein. The whey or the concentrated whey protein left after the recovery of the immunoglobulin can be utilized as an acid whey as it is or as a concentrated acid whey protein.
Bibliography:Application Number: JP19880254914