RECOVERY OF MONOCLONAL ANTIBODY

PURPOSE:To inexpensively recover the monoclonal antibody of high purity from the culture mixture by adjusting the culture mixture containing the monoclonal antibody to 6.5-8.5pH and treating the culture mixture with a cation exchange resin. CONSTITUTION:A culture mixture containing a monoclonal anti...

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Bibliographic Details
Main Authors YUASA HIDEO, OSUMI KAZUOKI, FUJIMOTO HIDEKI, HAGIWARA HIDEAKI, NAGAO JUNZO, HANE MOTOMU
Format Patent
LanguageEnglish
Published 02.10.1989
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPIRITS
VINEGAR
WINE
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Summary:PURPOSE:To inexpensively recover the monoclonal antibody of high purity from the culture mixture by adjusting the culture mixture containing the monoclonal antibody to 6.5-8.5pH and treating the culture mixture with a cation exchange resin. CONSTITUTION:A culture mixture containing a monoclonal antibody such as CLN-IgG produced by a human-human hybridoma CLN/SUZ H11 strains is adjusted in pH to 6.5-8.5. The culture mixture is treated with a cation-exchange resin having sulfonic or carboxylic acid groups to adsorb the monoclonal antibody. Then, a NaCl solution of a prescribed concentration is used as an eluent to elute the monoclonal antibody. The recovered monoclonal antibody is of high purity and can be used as a medicine.
Bibliography:Application Number: JP19880074559