POLYPEPTIDE HAVING CELL ADHESION ACTIVITY

• Main Authors: KIMIZUKA FUSAO, ODATE YOICHI, SUGAWARA YUKI, KATOU IKUNOSHIN, KINOSHITA RITSU
• Format: Patent
• Language: English
• Published: 21.08.1989
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PURPOSE:To facilitate these mass production of a polypeptide, by clarifying amino acid sequence of cell bonding domain in fibronectin. CONSTITUTION:It is clarified that polypeptide having 11.5KD (108 amino acid residue) and known as a polypeptide cell adhesion activity in human fibronectin (hereafter referred to as FN) has no cell adhesion action, whereas a peptide (Ala -Met ) having 283 amino acid residue obtained by extending N end of the above-mentioned polypeptide has adhesion activity equal to FN. Therefore, various investigation was carried out according to an amino acid sequence having N end domain. As a result, it turned out that a polypeptide having amino acid sequence expressed by formula I (X is amino acid sequence expressed by formula II or a sequence losing amino acid or peptide from the N-end thereof; Y is sequence having Cys or losing Cys) has extremely large cell adhesion activity.