FUSION PROTEIN OF SERUM ALBUMIN AND PHYSIOLOGICALLY ACTIVE PROTEIN

• Main Authors: TAKAHASHI KENICHI, FUJIYAMA SAKI
• Format: Patent
• Language: English; Japanese
• Published: 23.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide a method for efficiently producing a physiologically active protein, which has low levels of expression and/or activity when expressed as a recombinant protein normally using a host cell such as a CHO cell, as a highly active recombinant protein.SOLUTION: The present invention provides a fusion protein comprising human brain-derived neurotrophic factor, human nerve growth factor, neurotrophic factor, human neurotrophin-3 or human neurotrophin-4, and serum albumin (SA). Further, the fusion protein can be a conjugate with a transferrin antibody or the like for passage through the blood-brain barrier.SELECTED DRAWING: Figure 1 【課題】通常ではＣＨＯ細胞等の宿主細胞を用いて組換え蛋白質として発現させたときに，発現量が低く，及び／又は，活性が低い生理活性蛋白質を，活性の高い組換え蛋白質として効率よく製造することができる方法を提供する。【解決手段】ヒト脳由来神経栄養因子，ヒト神経成長因子，神経栄養因子，ヒトニューロトロフィン－３又はヒトニューロトロフィン－４と，血清アルブミン（ＳＡ）を含んでなる，融合蛋白質を提供する。更に，当該融合蛋白質は，血液脳関門を通過させるためにトランスフェリン抗体等との結合体とすることもできる。【選択図】図１