METHOD FOR MEASURING ACTIVITY OF ALKALINE PHOSPHATASE CONTAINED IN EXTRACELLULAR VESICLE, CALIBRATOR, AND CONJUGATE

To provide a calibrator for use in measurement of extracellular vesicles.SOLUTION: The foregoing problem is solved by acquiring an activity value of alkaline phosphatase contained in an extracellular vesicle in a sample, using a calibrator containing a conjugate in which a polypeptide having alkalin...

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Bibliographic Details
Main Authors TAMADA EIYA, ELNAZ NAKHAEI, ONISHI MASAMITSU, YAMASHITA KAZUTO, YANAGIDA MASATOSH, KIRIYAMA MARIA
Format Patent
LanguageEnglish
Japanese
Published 03.04.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:To provide a calibrator for use in measurement of extracellular vesicles.SOLUTION: The foregoing problem is solved by acquiring an activity value of alkaline phosphatase contained in an extracellular vesicle in a sample, using a calibrator containing a conjugate in which a polypeptide having alkaline phosphatase activity and a polypeptide including an amino acid sequence of a membrane protein present on the surface of the extracellular vesicle are covalently bound.SELECTED DRAWING: Figure 9 【課題】細胞外小胞の測定に用いられるキャリブレータを提供することを課題とする。【解決手段】アルカリフォスファターゼ活性を有するポリペプチドと、細胞外小胞が表面に有する膜タンパク質のアミノ酸配列を含むポリペプチドとが共有結合により結合した結合体を含むキャリブレータを用いて、試料中の細胞外小胞に含まれるアルカリフォスファターゼの活性値を取得することによって課題を解決する。【選択図】図９
Bibliography:Application Number: JP20220151929