METHODS OF MAKING ANTIBODIES

To provide methods of improving pairing of a heavy chain and a light chain of an antibody (such as a bispecific antibody) and also provide antibodies (e.g., bispecific antibodies) generated using such methods, libraries, and methods of screening such libraries.SOLUTION: A method of improving prefere...

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Bibliographic Details
Main Authors PAUL J CARTER, YIN YIYUAN, KAMAL KISHORE JOSHI
Format Patent
LanguageEnglish
Japanese
Published 05.03.2024
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:To provide methods of improving pairing of a heavy chain and a light chain of an antibody (such as a bispecific antibody) and also provide antibodies (e.g., bispecific antibodies) generated using such methods, libraries, and methods of screening such libraries.SOLUTION: A method of improving preferential pairing of a heavy chain and a light chain of an antibody is also provided, comprising the step of substituting at least one amino acid at position 94 of a light chain variable domain (VL) or position 96 of the VL, from a non-charged residue to a charged residue selected from the group consisting of aspartic acid (D), arginine (R), glutamic acid (E), and lysine (K), wherein the amino acid numbering is according to Kabat.SELECTED DRAWING: Figure 2 【課題】抗体（例えば、二重特異性抗体）の重鎖と軽鎖との対合を改善する方法を提供する。そのような方法、ライブラリ、およびそのようなライブラリをスクリーニングする方法を使用して生成された抗体（例えば、二重特異性抗体）も提供する。【解決手段】抗体の重鎖と軽鎖との優先的対合を改善する方法であって、軽鎖可変ドメイン（ＶＬ）の９４位またはＶＬの９６位の少なくとも１つのアミノ酸を、非荷電残基から、アスパラギン酸（Ｄ）、アルギニン（Ｒ）、グルタミン酸（Ｅ）およびリジン（Ｋ）からなる群から選択される荷電残基に置換するステップを含み、アミノ酸番号付けはＫａｂａｔに従っている、方法である。【選択図】図２
Bibliography:Application Number: JP20230203630