ヒト対象における医学的状態を同定する方法

• Format: Patent
• Language: Japanese
• Published: 07.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

本開示は、対象における医学的状態を検出するためのインビトロ及び非侵襲的方法を開示する。この方法は、試料から非常に小さな胚様幹細胞を濃縮して、該非常に小さな胚様幹細胞を含む混合物を得ることと、工程の混合物から核酸を得ることと、試料からの非常に小さな胚様幹細胞におけるＯｃｔ４Ａの発現レベルを分析するために核酸にアッセイを実施することと、試料からの非常に小さな胚様幹細胞におけるＯｃｔ４Ａの発現レベルを、対照試料におけるＯｃｔ４Ａの発現レベルと比較することと、を含む。本開示はまた、がんの発症を予測し、がんの存在を予測するための方法を提供する。がんを治療する方法も、本明細書に開示される。更に、試薬キット及び検出キットもまた開示される。 The present disclosure discloses an in-vitro and non-invasive method for detecting a medical condition in a subject. The method involves enriching very small embryonic like stem cells from the sample, to obtain a mixture comprising said very small embryonic like stem cells; obtaining nucleic acid from the mixture of step; performing an assay with the nucleic acid for analysing expression level of Oct4A in the very small embryonic like stem cells from the sample; and comparing the expression level of Oct4A in the very small embryonic like stem cells from the sample with an expression level of Oct4A in a control sample. The present disclosure also provides a method for predicting the onset of cancer and for predicting the presence of cancer. A method of treating cancer is also disclosed herein. Moreover, a reagent kit and a detection kit are also disclosed.