CELL CULTURE SUBSTRATE

• Main Author: KUNO TAKESHI
• Format: Patent
• Language: English; Japanese
• Published: 14.12.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

To provide a cell culture substrate that can form a cell aggregate, can reduce UV-induced cell damage, and ensures superior cell visibility and a method for producing the same.SOLUTION: A cell culture substrate has a substrate, and a polymer layer that coats at least part of the substrate surface. The polymer lacks the ability to adhere to cells or promote cell growth. The cell culture substrate includes the following regions (A) and (B) and has a yellowness of 10-100 as defined by JIS K 7373: 2006. (A) is a region with an area of 0.001-5 mm2, having the ability to adhere to cells or promote cell growth. (B) is a region adjacent to the (A) region, lacking the ability to adhere to cells or promote cell growth.SELECTED DRAWING: Figure 1 【課題】細胞凝集塊を形成可能であるとともに、紫外線による細胞へのダメージを低減可能であり、細胞の視認性に優れる細胞培養基材及びその製造方法を提供すること。【解決手段】基材と、前記基材の表面の少なくとも一部を被覆する高分子層とを備える細胞培養基材であって、前記高分子は細胞接着性又は細胞増殖性を有しないものであり、前記細胞培養基材は下記（Ａ）領域及び下記（Ｂ）領域を有し、ＪＩＳ Ｋ ７３７３：２００６で規定される黄色度が１０～１００である、細胞培養基材。（Ａ）細胞接着性及び細胞増殖性を有する面積０．００１～５ｍｍ２の島状の領域（Ｂ）前記（Ａ）領域に隣接し、細胞接着性又は細胞増殖性を有しない領域【選択図】図１