SEQUENCES FOR DETECTION AND IDENTIFICATION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) OF MREJ TYPE XXI

• Main Authors: CHRISTIAN MENARD, CELINE ROGER-DALBERT
• Format: Patent
• Language: English; Japanese
• Published: 18.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide compositions and methods for the detection and identification of Staphylococcus aureus strains harboring polymorphic SCCmec right extremity (MREP) type xxi sequences.SOLUTION: Provided is a composition for the detection of methicillin-resistant Staphylococcus aureus (MRSA) comprising MREJ type xxi nucleic acids, the S. aureus comprising a Staphylococcal cassette chromosome mec (SCCmec) element including a mecA homo log, the SCCmec cassette being inserted into S. aureus chromosomal DNA, thereby generating a polymorphic right extremity junction (MREJ) type xxi sequence, the sequence comprising polymorphic sequences from the right extremity and chromosomal DNA adjoining the polymorphic right extremity, the composition comprising a first amplification primer that is between 10 and 45 nucleotides in length and specifically hybridizes under standard PCR conditions to the polymorphic right extremity sequences of the MREJ type xxi nucleic acids.SELECTED DRAWING: None 【課題】多形ＳＣＣｍｅｃ右端接合部（ＭＲＥＰ）タイプｘｘｉ配列を有するＳｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ株を検出及び同定するための組成物を提供する。【解決手段】ＭＲＥＪタイプｘｘｉ核酸を含むメチシリン耐性Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓ（ＭＲＳＡ）を検出するための組成物であって、該Ｓ．ａｕｒｅｕｓは、ｍｅｃＡホモログを含むブドウ球菌カセット染色体ｍｅｃ（ＳＣＣｍｅｃ）エレメントを含み、該ＳＣＣｍｅｃカセットは、Ｓ．ａｕｒｅｕｓ染色体ＤＮＡ内に挿入されており、それによって多型右端接合部（ＭＲＥＪ）タイプｘｘｉ配列を生じ、該配列は、右端からの多型配列および該多型右端に隣接する染色体ＤＮＡを含み、該組成物は、長さが１０～４５ヌクレオチドの間であり、標準的なＰＣＲ条件下で該ＭＲＥＪタイプｘｘｉ核酸の該多型右端配列に特異的にハイブリダイズする第１増幅プライマーを含む、組成物である。【選択図】なし