EXTRACTION METHOD OF EFFICIENT PROTEIN, AND EFFICIENT PRODUCTION METHOD OF PROTEIN EXTRACT LIQUID

• Main Authors: NAGATO TAKASHI, AIBA SHINYA
• Format: Patent
• Language: English; Japanese
• Published: 09.08.2023
• Subjects: COFFEE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROTEIN COMPOSITIONS FOR FOODSTUFFS; TEA; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR SUBSTITUTES; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; WORKING-UP PROTEINS FOR FOODSTUFFS

To maintain pH and temperature of reaction mixture liquid containing enzyme and a protein-extracting subject within an appropriate range, and efficiently extract protein from the protein-extracting subject.SOLUTION: A protein extraction method of the present invention includes: a first mixing process of mixing electrolytic water having the temperature 30°C to 70°C and pH4 to 5, cellulase of the genus Aspergillus origin, and cellulase of the genus Trichoderma origin to obtain enzyme mixture liquid; a second mixing process of mixing the enzyme mixture liquid and protein-extracting subject to obtain reaction mixture liquid; and an extraction process of maintaining the temperature and pH of the reaction mixture liquid at 30°C to 70°C and 4 to 5 respectively and extracting protein from the protein-extracting subject, where the protein-extracting subject is any one or more kinds of tea leaves, used tea leaves, coffee beans, coffee chaff, or seeds.SELECTED DRAWING: None 【課題】酵素とタンパク質抽出対象物を含む反応混合液のｐＨ、温度を適切な範囲に維持して、タンパク質抽出対象物からタンパク質を効率的に抽出する。【解決手段】本発明のタンパク質抽出方法は、温度３０℃～７０℃、かつ、ｐＨ４～５の電解水と、アスペルギルス属菌起源のセルラーゼ、および、トリコデルマ属菌起源のセルラーゼと、を混合して、酵素混合液を得る第１混合工程と、前記酵素混合液と、タンパク質抽出対象物と、を混合して反応混合液を得る第２混合工程と、前記反応混合液の温度を３０℃～７０℃、かつ、ｐＨを４～５に維持して、前記タンパク質抽出対象物からタンパク質を抽出する抽出工程と、を含み、前記タンパク質抽出対象物は、茶葉、茶殻、コーヒー豆、コーヒー殻、または、種実類のいずれか１種以上である。【選択図】なし