CHLORAMPHENICOL DETOXIFIED ENZYME HAVING IMPROVED THERMAL STABILITY, AND METHOD FOR PRODUCING THE SAME

To provide a chloramphenicol detoxified enzyme having improved thermal stability, and a method for producing the enzyme.SOLUTION: The problem is solved by substituting 17th histidine residue of an amino acid sequence shown by sequence number 1 into one kind of amino acid residue selected from valine...

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Bibliographic Details
Main Authors HOTANI MEGUMI, INOUE MUNENOBU
Format Patent
LanguageEnglish
Japanese
Published 24.07.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:To provide a chloramphenicol detoxified enzyme having improved thermal stability, and a method for producing the enzyme.SOLUTION: The problem is solved by substituting 17th histidine residue of an amino acid sequence shown by sequence number 1 into one kind of amino acid residue selected from valine residue, alanine residue and proline residue, and/or substituting 34th asparagine residue of an amino acid sequence shown by sequence number 1 into serine residue or alanine residue, and/or substituting 134th phenylalanine residue into alanine residue or methionine residue, and/or substituting 157th aspartic acid residue into one kind of amino acid residue selected from cysteine residue, alanine residue, glycine residue, asparagine residue, histidine residue and threonine residue, and/or substituting 198th glycine residue into one kind of amino acid residue selected from leucine residue, cysteine residue, threonine residue, valine residue, asparagine residue, alanine residue, isoleucine residue and serine residue, and by producing the chloramphenicol detoxified enzyme by using a coliform bacillus.SELECTED DRAWING: None 【課題】 熱安定性が向上したクロラムフェニコール無毒化酵素およびその製造方法を提供すること。【解決手段】配列番号１に示されるアミノ酸配列の１７番目のヒスチジン残基をバリン残基、アラニン残基およびプロリン残基から選ばれる１種類のアミノ酸残基に置換すること、および／または、配列番号１に示されるアミノ酸配列の３４番目のアスパラギン残基をセリン残基またはアラニン残基に置換すること、および／または、１３４番目のフェニルアラニン残基をアラニン残基またはメチオニン残基に置換すること、および／または、１５７番目のアスパラギン酸残基をシステイン残基、アラニン残基、グリシン残基、アスパラギン残基、ヒスチジン残基およびスレオニン残基から選ばれる１種類のアミノ酸残基に置換すること、および／または、１９８番目のグリシン残基をロイシン残基、システイン残基、スレオニン残基、バリン残基、アスパラギン残基、アラニン残基、イソロイシン残基およびセリン残基から選ばれる１種類のアミノ酸残基に置換すること、加えて、大腸菌を用いて当該クロラムフェニコール無毒化酵素を製造することにより、前記課題を解決する。【選択図】 なし
Bibliography:Application Number: JP20220167553