PEPTIDE THIOESTER AND NOVEL METHOD FOR PRODUCING PEPTIDE

To provide an efficient and versatile peptide thioester, and a novel method for producing peptides.SOLUTION: A method for producing a peptide thioester includes the steps of: (1) providing a peptide thioester with a CGC triplet at the C-terminus; (2) effecting a transfer between the SH group of the...

Full description

Saved in:
Bibliographic Details
Main Authors MURASE TAKEFUMI, KAJIWARA YASUHIRO, OKAMOTO AKIRA, MAKI YUTA, NISHIKAWA RIE, NOMURA KOTA, AMAZAKI YOKO
Format Patent
LanguageEnglish
Japanese
Published 19.07.2023
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
Online AccessGet full text

Cover

More Information
Summary:To provide an efficient and versatile peptide thioester, and a novel method for producing peptides.SOLUTION: A method for producing a peptide thioester includes the steps of: (1) providing a peptide thioester with a CGC triplet at the C-terminus; (2) effecting a transfer between the SH group of the C-terminal cysteine in the CGC triplet and the carbonyl group of a glycine to obtain an R-X-CG-thioester; and (3) in the R-X-CG-thioester, effecting a transfer between the SH group of the cysteine and the carbonyl group of the X, and a transfer between the amino group of the cysteine and the thiol group of the glycine to obtain a peptide thioester. A method for producing peptides using the peptide thioester produced by the method is also provided.SELECTED DRAWING: None 【課題】本発明は、効率的かつ汎用性の高い、ペプチドチオエステルおよびペプチドの新規な製造方法を提供することを目的とする。【解決手段】（１）Ｃ末端にＣＧＣトリプレットを有するペプチドチオエステルを準備する工程、（２）ＣＧＣトリプレットにおけるＣ末端システインのＳＨ基とグリシンのカルボニル基との間で転移を生じさせ、Ｒ－Ｘ－ＣＧ－チオエステルを得る工程、および（３）前記Ｒ－Ｘ－ＣＧ－チオエステルにおいて、システインのＳＨ基とＸのカルボニル基との転移と、システインのアミノ基とグリシンのチオール基との転移とを生じさせて、ペプチドチオエステルを得る工程を含む、ペプチドチオエステルの製造方法、および当該方法により製造されたペプチドチオエステルを用いたペプチドの製造方法が提供される。【選択図】なし
Bibliography:Application Number: JP20230077093