METHOD FOR CULTURE OF CELLS

• Main Authors: IKEYA MAKOTO, KAMIYA YAYOI
• Format: Patent
• Language: English; Japanese
• Published: 04.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide a method for producing neural crest cells that maintain multipotency.SOLUTION: As a technique for proliferating neural crest cells without reducing differentiation capacity, provided is a method for producing neural crest cells, comprising the steps of: (1) obtaining neural crest cells; and (2) suspension-culturing the neural crest cells in a medium comprising a GSK3β inhibitor and a basic fibroblast growth factor (bFGF), wherein the medium comprises the GSK3β inhibitor with a concentration that exhibits an effect equivalent to that exhibited by CHIR99021 with a concentration of higher than 1 μM and lower than 5 μM.SELECTED DRAWING: None 【課題】多能性を維持した神経堤細胞の製造方法を提供する。【解決手段】分化能を低下させることなく神経堤細胞を増殖させるための技術として、（１）神経堤細胞を得る工程と、（２）ＧＳＫ３β阻害剤及び塩基性線維芽細胞成長因子（ｂＦＧＦ）を含む培地中で神経堤細胞を浮遊培養する工程であって、１μＭを超え５μＭ未満の濃度のＣＨＩＲ９９０２１が示す効果と同等の効果を示す濃度のＧＳＫ３β阻害剤を、該培地が含む工程を含む、神経堤細胞の製造方法を提供する。【選択図】なし