CELL PROCESSING METHOD AND CELL PROCESSING DEVICE

• Main Authors: HIROSE FUTOSHI, MORITA YUKI, YAMAUCHI SACHIKO, ITO SADANOBU, ORIHARA TATSUMASA
• Format: Patent
• Language: English; Japanese
• Published: 21.06.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide a cell processing method and a cell processing device, which have high cell processing efficiency.SOLUTION: Provided is a cell processing method comprising a step of passing a liquid containing cells from a channel to an orifice by pressure application means. The orifice is connected to the channel to constrict flow from the channel. The area of the cross section passing through the center of the orifice and perpendicular to the flow direction of the liquid has at least one of a portion where no change occurs in the area from the entrance to the exit of the orifice and a portion where the area increases from the entrance to the exit of the orifice. Also provided is a cell processing device comprising: an orifice forming member having the orifice; the channel; and the pressure application means.SELECTED DRAWING: Figure 1A 【課題】細胞加工の効率が高い細胞加工方法および細胞加工装置を提供する。【解決手段】細胞を含む液体を、圧力付与手段によって流路からオリフィスに通過させる工程を有する細胞加工方法であって、前記オリフィスは、前記流路からの流れを狭窄するように前記流路と接続されており、かつ、前記オリフィスの中心を通る前記液体の流れ方向に垂直な断面の面積が、前記オリフィスの入口から出口に向かって変化しない部分、および、前記面積が、前記オリフィスの入口から出口に向かって増加する部分の少なくともいずれか一方を有する、ことを特徴とする細胞加工方法、および、前記オリフィスを有するオリフィス形成部材と、前記流路と、前記圧力付与手段とを有する、ことを特徴とする細胞加工装置。【選択図】図１Ａ