METHOD FOR DISTINGUISHING SENILE PIGMENT FRECKLE FROM DNA METHYLATION CHANGES AND RNA EXPRESSION CHANGES

• Main Authors: INOUE DAIGO, ONO RYUSUKE, SHIBATA TAKAKO, NISHIGORI CHIKAKO, TSUTSUI DAIKI, KOIKE SAYA, OBA HIROSHI, KUNISADA MITSURU
• Format: Patent
• Language: English; Japanese
• Published: 25.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

To provide a screening method for a gene involved in blemish formation and a screening method for a substance that inhibits the formation of blemishes or a substance that ameliorates blemishes.SOLUTION: A method comprises: a step of preparing epidermal cells of an isolated pigment-enhanced site; a step of isolating DNA and RNA from the epidermal cells; a step of analyzing the DNA by whole-genome bisulfite sequencing; a step of analyzing the RNA by RNA-seq; and a step of selecting a gene using, as an index, an increase or decrease in DNA methylation compared to a prescribed standard and an increase or decrease of RNA expression compared to a prescribed standard. The gene identified by the method is used to further screen for substances that inhibit the formation of blemishes or ameliorate blemishes.SELECTED DRAWING: None 【課題】シミの形成にかかわる遺伝子のスクリーニング方法、およびシミの形成を阻害する物質またはシミを改善する物質のスクリーニング方法の提供。【解決手段】単離された色素増強部位の表皮細胞を用意する工程、表皮細胞からDNAおよびRNAを単離する工程、DNAを全ゲノムバイサルファイトシーケンス法により分析する工程、RNAをRNA-seq法により分析する工程、およびDNAメチル化が所定の標準と比較して亢進もしくは減少しており、かつRNA発現が所定の標準と比較して増加もしくは低下していることを指標として遺伝子を選択する工程を含む、方法が提供される。上記方法により同定された遺伝子を用いて、シミの形成を阻害する物質またはシミを改善する物質のスクリーニングをさらに行う。【選択図】なし