METHOD FOR DETERMINING TYPE OF SARS-COV-2, PROBE SET USED IN METHOD THEREOF, AND PRIMER PROBE SET USED IN METHOD THEREOF

• Main Authors: YAMASHITA KEITA, MIYAMOTO SHIGEHIKO
• Format: Patent
• Language: English; Japanese
• Published: 14.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide methods for determining the type of SARS-CoV-2, i.e., methods that can determine many types using a limited number of probes.SOLUTION: One or more embodiments of the present invention provide a method for determining the type of SARS-CoV-2, the method comprising contacting a nucleic acid containing a base sequence containing N (N is an integer of 2 or more) mutation positions in the spike protein gene derived from the test virus with N kinds of probes to detect the presence or absence of hybridization between the nucleic acid and each of the N kinds of probes, and the n-th probe, which is each of the N kinds of probes, can hybridize with only one of the gene in which the n-th mutation position is a wild-type base and the gene in which the n-th mutation position is a mutant base.SELECTED DRAWING: None 【課題】本明細書は、ＳＡＲＳ－ＣｏＶ－２の型を判定する方法であって、限られた種類のプローブを用い、多くの型の判定が可能な方法を開示する。【解決手段】本発明の一以上の実施形態はＳＡＲＳ－ＣｏＶ－２の型を判定する方法であって、被検ウイルス由来のスパイクタンパク質遺伝子におけるＮ（Ｎは２以上の整数）個の変異位置を含む塩基配列を含む核酸と、Ｎ種のプローブとを接触させ、前記核酸と、前記Ｎ種のプローブのそれぞれとのハイブリダイゼーションの有無を検出することを含み、前記Ｎ種のプローブの各々である第ｎプローブは、第ｎ変異位置が野生型塩基である前記遺伝子、及び、第ｎ変異位置が変異型塩基である前記遺伝子の一方のみとハイブリダイズすることができる。【選択図】なし