PRODUCTION OF METABOLITES USING PEPTIDE-TAGGED PROTEINS

• Main Authors: OIWA KIYOKA, KOIKE KAZUYOSHI
• Format: Patent
• Language: English; Japanese
• Published: 18.01.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide methods of efficient fermentative production of metabolic products.SOLUTION: Disclosed is a method for producing a metabolite comprising the steps of: culturing a microorganism in a culture medium containing a carbon source; and collecting the metabolite from the microorganism or the culture medium, where the microorganism has been modified to express one or more enzymes involved in the biosynthesis of the metabolite as a fusion protein tagged with the following peptide tag: X1(PY1)qPZ1 (I), X2mZ2nPU1q' (II), X3m'Z3n'U2q'' (III), X4m''(JY2n'')q'''Z4r (IV).SELECTED DRAWING: Figure 9 【課題】代謝産物を効率よく発酵生産するための方法を提供すること。【解決手段】炭素源を含む培地で微生物を培養して代謝産物を生成させる工程、および、微生物菌体又は培地から代謝産物を回収する工程、を含む代謝産物の製造方法であって、前記微生物は、前記代謝産物の生合成に関与する１種以上の酵素が下記のペプチドタグが付加された融合タンパク質として発現するように改変されていることを特徴とする、前記製造方法。Ｘ1(ＰＹ1)qＰＺ1・・・（I）Ｘ2ｍＺ2ｎＰＵ1ｑ'・・・（II）Ｘ3ｍ'Ｚ3ｎ'Ｕ2ｑ''・・・（III）Ｘ4m''(ＪＹ2n'')q'''Ｚ4r・・・（IV）【選択図】図９