反復性がん突然変異を標的とする免疫原性組成物およびその使用の方法

• Format: Patent
• Language: Japanese
• Published: 10.01.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

【課題】反復性がん突然変異を標的とする免疫原性組成物およびその使用の方法の提供。【解決手段】がん関連タンパク質に由来する１つまたは複数の抗原性ペプチド（例えばＰＥＳＴ含有ペプチドに融合したもの）を含む組換え融合ポリペプチドが本明細書において提供される。抗原性ペプチドは、反復性がん突然変異を含む抗原性ペプチド、ヘテロクリティック突然変異を含む抗原性ペプチド、またはユビキチンタンパク質に融合した抗原性ペプチドのうちの１つもしくは複数またはすべてを含み得る。例えば、２つまたはそれよりも多くの抗原性ペプチド（例えばＰＥＳＴ含有ペプチドに融合したもの）を含む組換え融合ポリペプチドであって、各抗原性ペプチドが、反復性がん突然変異を含み、抗原性ペプチドのうちの少なくとも２つが、同じがん関連タンパク質の断片である、組換え融合ポリペプチドが、本明細書において提供される。【選択図】なし Provided herein are recombinant fusion polypeptides comprising one or more antigenic peptides (e.g., fused to a PEST-containing peptide) from cancer-associated proteins. The antigenic peptides can comprise one or more or all of an antigenic peptide comprising a recurrent cancer mutation, an antigenic peptide comprising a heteroclitic mutation, or an antigenic peptide fused to a ubiquitin protein. For example, provided herein are recombinant fusion polypeptides comprising two or more antigenic peptides (e.g., fused to a PEST-containing peptide), wherein each antigenic peptide comprises a recurrent cancer mutation, and wherein at least two of the antigenic peptides are fragments of the same cancer-associated protein. Also provided are nucleic acids encoding such fusion polypeptides, recombinant bacteria or Listeria strains comprising such fusion polypeptides or such nucleic acids, and cell banks comprising such recombinant bacteria or Listeria strains. Also provided herein are methods of generating such fusion polypeptides, such nucleic acids, and such recombinant bacteria or Listeria strains. Also provided are immunogenic compositions, pharmaceutical compositions, and vaccines comprising such fusion polypeptides, such nucleic acids, or such recombinant bacteria or Listeria strains. Also provided are methods of inducing an anti-tumor-associated-antigen immune response in a subject, methods of inducing an anti-tumor or anti-cancer immune response in a subject, methods of treating a tumor or cancer in a subject, methods of preventing a tumor or cancer in a subject, and methods of protecting a subject against a tumor or cancer using such recombinant fusion polypeptides, nucleic acids, recombinant bacteria or Listeria strains, immunogenic compositions, pharmaceutical compositions, or vaccines.