METHOD FOR DETERMINING RISK OF DEVELOPING CARDIOVASCULAR DISEASE IN SUBJECT, METHOD FOR DETERMINING CALCIFICATION OF BLOOD IN SUBJECT, AND INSPECTION KIT

• Main Authors: TAMADA EIYA, ELNAZ NAKHAEI, ONISHI MASAMITSU, KIRIYAMA MARIA
• Format: Patent
• Language: English; Japanese
• Published: 06.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

To provide a method for determining the risk of developing a cardiovascular disease in a subject more easily by using a blood sample.SOLUTION: The present invention relates to a method for determining the risk of developing a cardiovascular disease in a subject, the method including the steps of: capturing an extracellular vesicle derived from a sample cell collected from a subject on a solid phase; and measuring the alkaline phosphatase activity of the extracellular vesicle. The result of measurement of the alkaline phosphatase activity serves as an indicator of the risk of developing the cardiovascular disease in the subject.SELECTED DRAWING: Figure 12A 【課題】本発明は、血液試料を用いることで、より簡便に被検者における心血管疾患の発症リスクを判定する方法を提供することを課題とする。【解決手段】被検者から採取された血液試料に由来する細胞外小胞を固相上に捕捉する工程と、前記細胞外小胞のアルカリフォスファターゼ活性を測定する工程と、を含み、前記アルカリフォスファターゼ活性の測定結果が、前記被検者における心血管疾患の発症リスクの指標となる、前記被検者における心血管疾患の発症リスクの判定方法により、課題を解決する。【選択図】図１２Ａ