METHODS FOR DETERMINING LOW AMYLOSE CONTENT RICE PLANT HAVING MUTATION IN SART-1

• Main Authors: TAKEUCHI YOSHINOBU, YAMAUCHI UTAKO, ANDO IKUO, HORI KIYOSUMI, KUROKI MAKOTO, KUNO YOKO, HIRABAYASHI HIDESUKE, SUZUKI KEITARO, ISHII TAKURO
• Format: Patent
• Language: English; Japanese
• Published: 03.10.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

To provide methods and kits for determining or selecting rice plant of low amylose trait.SOLUTION: Disclosed is a method for determining or selecting strains of rice plant of low amylose trait comprising a step of determining whether a subject rice plant has a gene encoding a mutant SART-1 protein or not, where the mutant SART-1 protein comprises a mutation selected from the group consisting of: (a) an insertion mutation in four repetitive sequences of glutamic acid and lysine (EK) from position 56 to position 63 of the wild type SART-1 protein to increase the repetition number of EK by one; (b) methionine at position 586 of the wild type SART-1 protein is substituted with an amino acid selected from threonine, asparagine, cysteine, glutamine, serine and tyrosine; and (c) serine at position 745 of the wild type SART-1 protein is substituted with an amino acid selected from threonine, cysteine, asparagine, glutamine, serine and tyrosine.SELECTED DRAWING: None 【課題】低アミロース形質のイネを判定又は選別するための方法及びキットを提供する。【解決手段】低アミロース形質のイネを判定又は選抜する方法であって、対象のイネが、変異型SART-1タンパク質をコードする遺伝子を有するか否かを検出するステップを含み、前記変異型SART-1タンパク質が、以下：（a）野生型SART-1タンパク質の56位から63位におけるグルタミン酸及びリジン（EK）の4回反復配列中に、EKの反復数が1回増加する挿入変異、（b）野生型SART-1タンパク質の586位のメチオニンが、スレオニン、アスパラギン、システイン、グルタミン、セリン及びチロシンから選択されるアミノ酸に置換する変異、並びに（c）野生型SART-1タンパク質の745位のセリンが、システイン、アスパラギン、グルタミン、スレオニン及びチロシンから選択されるアミノ酸に置換する変異からなる群より選択される変異を含む、方法。【選択図】なし