MUTANT BEETLE LUCIFERASE, GENE, RECOMBINANT VECTOR, TRANSFORMANT, AND METHOD FOR PRODUCING MUTANT BEETLE LUCIFERASE

To provide a mutant beetle luciferase that is less susceptible to luminescence inhibition by NaCl.SOLUTION: Provided is a mutant beetle luciferase that has a mutation in which the amino acid corresponding to phenylalanine at position 294 in the amino acid sequence of wild-type Heike firefly lucifera...

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Bibliographic Details
Main Authors SHIMOMURA AI, NODA KENICHI, YAHATA SATOSHI
Format Patent
LanguageEnglish
Japanese
Published 26.09.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:To provide a mutant beetle luciferase that is less susceptible to luminescence inhibition by NaCl.SOLUTION: Provided is a mutant beetle luciferase that has a mutation in which the amino acid corresponding to phenylalanine at position 294 in the amino acid sequence of wild-type Heike firefly luciferase is valine, or a mutation in which the amino acid corresponding to aspartic acid at position 533 is valine, in the amino acid sequence encoding wild-type beetle luciferase, the luminescence intensity of the luciferin-luciferase luminescence reaction in 0.9 mass% of a NaCl solution being 50% or more of that in a NaCl-free solution.SELECTED DRAWING: None 【課題】ＮａＣｌによる発光阻害の影響を受けにくい変異型甲虫ルシフェラーゼの提供。【解決手段】野生型甲虫ルシフェラーゼをコードするアミノ酸配列において、野生型ヘイケボタルルシフェラーゼのアミノ酸配列における、２９４位のフェニルアラニンに相当するアミノ酸がバリンである変異、又は、５３３位のアスパラギン酸に相当するアミノ酸がバリンである変異を有しており、０．９質量％のＮａＣｌ溶液中におけるルシフェリン－ルシフェラーゼ発光反応による発光強度が、ＮａＣｌ非含有溶液中の５０％以上である、変異型甲虫ルシフェラーゼ。【選択図】なし
Bibliography:Application Number: JP20210040545