CELL CULTURE PROCESS FOR RSV F PROTEIN PRODUCTION

• Main Authors: BHANU CHANDRA MULUKUTLA, MICHAELA EVELINA JACOBS, CAMERON ALBERT HARRINGTON, SHELBY HUTCHINS BREEN, JASON ARNOLD LOTVIN, DAVID STEAD, MADHURESH SUMIT
• Format: Patent
• Language: English; Japanese
• Published: 14.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

To provide a method for producing an RSV F protein trimer by a fed-batch cell culture method.SOLUTION: Provided is a method comprising a temperature transition. The temperature is shifted to a lower temperature between about 30.0°C and about 32.0°C, preferably about 31.0°C.SELECTED DRAWING: Figure 2 【課題】ＲＳＶ Ｆタンパク質三量体を流加細胞培養法で生産するための方法を提供すること。【解決手段】方法は、温度を、約３０．０℃～約３２．０℃の間のより低い温度、好ましくは、約３１．０℃に移行させる、温度移行を含む。【選択図】図２