SIMPLE GENETIC ANALYSIS TECHNIQUE ALLOWING VISUAL DETERMINATION USING NANOPARTICLE

• Main Authors: NAKAZATO TETSUYA, TANI HIDENORI, YAMAGUCHI MASAHIRO, KURATA SHINYA
• Format: Patent
• Language: English; Japanese
• Published: 18.02.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide a genetic test technique that (1) has a simple analysis process, (2) disallows the opening of a reaction tube after gene amplification, and (3) allows the specific detection of only a target gene and does not require expensive analyzing devices.SOLUTION: A method for detecting a test-target substance includes: a step in which nanoscale particles ("nanoparticles") having molecules having affinity with a test-target substance ("affinity molecules") labeled and having an average particle size of 1 nm or more and less than 1000 nm are reacted with the test-target substance in solution; a step in which the agglutination of nanoparticles is caused to be formed by a crosslinking reaction through the test-target substance; and a step in which a decrease in the turbidity of the solution due to the precipitation of the nanoparticles associated with the formation of the agglutination is measured, thus the presence or absence of the test-target substance is determined.SELECTED DRAWING: Figure 4 【課題】(1)シンプルな解析工程、(2)遺伝子増幅後は反応チューブを非開放、(3)標的遺伝子のみを特異的に検出可能を担保しつつ、高額な解析装置を必要としない遺伝子検査技術を提供すること。【解決手段】核酸プローブを複数標識した平均粒子径が１ ｎｍ以上かつ１０００ ｎｍ未満であるナノスケールの粒子を溶液中で標的遺伝子と反応させ、核酸プローブと標的遺伝子間の架橋反応を発生させることにより、ナノ粒子の凝集体を形成させ、凝集体形成に伴うナノ粒子の沈殿による溶液の濁度低下を測定することにより、標的遺伝子の有無を判定することを含む、標的遺伝子の検出方法。【選択図】図４