METHOD FOR PRODUCING NUCLEIC ACID AND COMPOSITION FOR SILANE COUPLING TREATMENT

• Main Authors: ISHIYAMA TAKESHI, TANAKA KENTA, OSHIRO IKUYA
• Format: Patent
• Language: English; Japanese
• Published: 18.11.2021
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; CHEMISTRY; COMPOUNDS THEREOF; DERIVATIVES THEREOF; INORGANIC CHEMISTRY; METALLURGY; NON-METALLIC ELEMENTS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SUGARS

To provide a method for producing a nucleic acid by using an inorganic substrate having an amino group introduced thereto so as to allow an increase in the purity and yield, and a composition for silane coupling treatment useful for the synthesis of the inorganic substrate.SOLUTION: The present invention discloses a method for producing a nucleic acid, by the phosphoroamidite solid phase synthesis, with an inorganic substrate having a silyl group introduced into an inorganic oxide having a pore size of 20 nm or more with a composition for silane coupling treatment, which is a mixture of a silane represented by formula (i-1) or the like with at least one organic acid selected from a specific group.SELECTED DRAWING: None 【課題】純度・収率をより高めることができるアミノ基が導入された無機基材を用いた、核酸の製造方法の提供、および該無機基材の合成に有用なシランカップリング処理用組成物の提供。【解決手段】式（ｉ−１）等で表されるシランと、特定の群から選ばれる少なくとも１つの有機酸を混合して得られるシランカップリング処理用組成物を用いて、細孔径として２０ｎｍ以上を有する無機酸化物へ、シリル基を導入することを特徴とする無機基材を用いて、ホスホロアミダイト固相合成法により、核酸を製造する方法。【選択図】なし