BIOLOGICAL SAMPLE ANALYSIS METHOD

• Main Authors: TSUJITA KOJI, ITONAGA MAKOTO
• Format: Patent
• Language: English; Japanese
• Published: 02.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

To provide a biological sample analysis method with which it is possible to analyze exosome inclusions and measure the number of pieces of exosome with one biological sample.SOLUTION: An exosome to which a first and a second bead are bound is recovered from a buffer solution that includes the exosome to which the first and second beads are bound. A first antibody that specifically binds to a first antigen associated with a first disease is fixed to the surface of the first bead. A second antibody that specifically binds to a second antigen associated with a second disease is fixed to the surface of the second bead. The first bead is separated from the exosome, and the exosome to which the second bead is bound is recovered. The exosome to which the second bead is bound is dissolved and the second bead and exosome inclusions are recovered (S19 to S20). The exosome inclusions are analyzed (S21), and the number of second beads is counted (S22).SELECTED DRAWING: Figure 1B 【課題】１つの生体試料で、エクソソームの内包物を分析し、エクソソームの個数を計測することができる生体試料分析方法を提供する。【解決手段】第１のビーズと第２のビーズとが結合したエクソソームを含む緩衝液から、第１のビーズと第２のビーズとが結合したエクソソームが回収される。第１のビーズの表面には、第１の疾患に関連した第１の抗原と特異的に結合する第１の抗体が固定されている。第２のビーズの表面には、第２の疾患に関連した第２の抗原と特異的に結合する第２の抗体が固定されている。エクソソームから第１のビーズが切り離され、第２のビーズが結合したエクソソームが回収される。第２のビーズが結合したエクソソームが溶解処理されて、第２のビーズとエクソソームの内包物とが回収される（Ｓ１９及びＳ２０）。エクソソームの内包物が分析され（Ｓ２１）、第２のビーズの個数が計測される（Ｓ２２）。【選択図】図１Ｂ