MODIFICATION METHOD OF ANTIBODY, AND MODIFIED ANTIBODY HAVING IMPROVED FUNCTIONAL PROPERTIES

• Main Authors: URECH DAVID, LEONARDO BORRAS
• Format: Patent
• Language: English; Japanese
• Published: 19.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide a method for using analysis based on a sequence and rational strategy in order to modify and improve structure characteristics and biophysical properties which are stability, solubility and/or antigen binding affinity, for example, of an immunobinder which is particularly a single chain antibody (scFv).SOLUTION: A method for engineering an immunobinder which is particularly an scFv by performing 1 or more substitutions at an amino acid position determined by analysis of database of a selected stable scFv sequence, in which preferable amino acid residue is determined for substitution.SELECTED DRAWING: None 【課題】イムノバインダーの、特に単鎖抗体（ｓｃＦｖ）の構造特性及び生物物理学的特性、例えば安定性、可溶性及び／又は抗原結合親和性を改変及び改善するために、配列に基づく分析及び合理的なストラテジーを使用する方法を提供する。【解決手段】イムノバインダー、特にｓｃＦｖを、選択された安定なｓｃＦｖ配列のデータベースの分析により割り出されたアミノ酸位置での１もしくはそれより多くの置換を行うことによりエンジニアリングする方法において、置換のために好ましいアミノ酸残基が割り出されている前記方法である。【選択図】なし