METHOD FOR PROLIFERATING ERYTHRONIUM JAPONICUM DECNE. BY TISSUE CULTURE

• Main Authors: HONDA KAZUSHIGE, KATO NAOMIKI, IWAMA NAOKO, TSUGAWA HIDEHITO
• Format: Patent
• Language: English; Japanese
• Published: 05.08.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

To provide methods for proliferating Erythronium japonicum Decne. plants in a short period of time and stably by tissue culture using materials that do not load or damage an ecosystem.SOLUTION: Provided is a method for proliferating Erythronium japonicum Decne. plants by tissue culture, comprising: a step of culturing ultraimmature seeds of Erythronium japonicum Decne. in a medium for inducing adventitious embryos, and inducing adventitious embryo tissue; a primary proliferation step of culturing at 18°C or lower the induced adventitious embryo tissue in a medium for proliferating adventitious embryo, and forming a tissue containing adventitious bud primordium; and a secondary proliferation step of dividing the tissue containing the formed adventitious bud primordium, further culturing the divided mass at 10 to 18°C, and proliferating the tissue containing adventitious bud primordium.SELECTED DRAWING: Figure 6 【課題】生態系への負荷やダメージがない材料を用い、組織培養によってカタクリ属植物を短期間でかつ安定的に増殖させる方法を提供すること。【解決手段】カタクリ属植物の超未熟種子を不定胚誘導用培地にて培養し、不定胚組織を誘導する工程、誘導した不定胚組織を不定胚増殖用培地にて１８℃以下で培養し、不定芽原基を含む組織を形成する一次増殖工程、及び形成された不定芽原基を含む組織を分割し、分割塊を不定胚増殖用培地にて１０〜１８℃でさらに培養し、不定芽原基を含む組織を増殖する二次増殖工程を含む、カタクリ属植物の組織培養による増殖方法。【選択図】図６