SYSTEMS AND METHODS FOR DETECTING CELLS USING ENGINEERED TRANSDUCTION PARTICLES

• Main Authors: SHAUNAK ROY, LEONARDO M TEIXEIRA, DAMIAN S MATTHEWS, DIEGO ARIEL REY, RYAN C GRISWOLD, BRUCE J RICHARDSON, RICK V STELLMACHER, KENNETH G OLSON, VICTOR H YEE
• Format: Patent
• Language: English; Japanese
• Published: 10.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide a method for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles.SOLUTION: The method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.SELECTED DRAWING: Figure 92 【課題】遺伝子操作した形質導入粒子を使用して標的細胞（例えば、細菌）を検出及び／又は同定するための方法を提供する。【解決手段】ある量の形質導入粒子を試料内に混合することを含む。形質導入粒子は、標的細胞と関連付けられる。形質導入粒子は、非複製的であり、標的細胞に一連のレポーター分子を生成させるように配合される核酸分子を含むように遺伝子操作される。試料及び形質導入粒子は、標的細胞が試料中に存在するときに一連のレポーター分子を発現するように維持される。レポーター分子の量と関連付けられる信号が受け取られる。いくつかの実施形態において、信号の大きさは、所定の量を超える形質導入粒子の量とは無関係である。【選択図】図９２