METHOD FOR PRODUCING SEQUENCING LIBRARY

To provide a sequencing library that gives reduced sequencing errors.SOLUTION: Provide is a method for producing a sequencing library that comprises: fragmenting a sample DNA; and treating the prepared sample DNA fragments with a single-strand specific nuclease to remove single-strand moieties from...

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Bibliographic Details
Main Authors MATSUMURA SHOJI, OTSUBO YUKI
Format Patent
LanguageEnglish
Japanese
Published 27.05.2021
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Summary:To provide a sequencing library that gives reduced sequencing errors.SOLUTION: Provide is a method for producing a sequencing library that comprises: fragmenting a sample DNA; and treating the prepared sample DNA fragments with a single-strand specific nuclease to remove single-strand moieties from the fragments.SELECTED DRAWING: None 【課題】シーケンシングエラーを低減させるシーケンシング用ライブラリの提供。【解決手段】サンプルDNAを断片化すること;及び、調製したサンプルDNAの断片を1本鎖特異的ヌクレアーゼで処理し、該断片から1本鎖部分を除去すること、を含むシーケンシング用ライブラリの調製方法。【選択図】なし
Bibliography:Application Number: JP20200077622