COMPOSITIONS AND METHODS COMPRISING THERMOLYSIN PROTEASE VARIANTS

• Main Authors: DANIEL ESTEBAN TORRES PAZMINO, YAO JIAN, HARM MULDER, FRITS GOEDEGEBUUR, DAVID A ESTELL, VIKTOR YURYEVICH ALEKSEYEV, BOTT RICHARD R, LILIA MARIA BABE
• Format: Patent
• Language: English; Japanese
• Published: 13.05.2021
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

To provide thermolysin enzymes, nucleic acids encoding the same, and compositions and methods related to the production and use thereof.SOLUTION: Provided are a thermolysin enzyme variant or an active fragment thereof comprising an amino acid substitution for a parent thermolysin enzyme having at least 90% sequence identity with an amino acid sequence consisting of a particular sequence, the substitution occurring at a productive position, the productive position and the amino acid substitution being selected from the group consisting of Q283E, A180E, I244T and T48E, and the amino acid positions of the thermolysin variant being numbered by correspondence with the amino acid sequence of thermolysin shown in an amino acid sequence consisting of the above particular sequence; and a cleaning composition comprising at least one thermolysin enzyme variant.SELECTED DRAWING: None 【課題】サーモリシン酵素、サーモリシン酵素をコードする核酸、並びにその製造及び使用に関する組成物及び方法を提供する。【解決手段】特定の配列からなるアミノ酸配列と少なくとも９０％の配列同一性を有する親酵素サーモリシンに対するアミノ酸の置換を含むサーモリシン酵素変異体又はその活性断片であって、前記置換は生産的位置で生じ、前記生産的位置及びアミノ酸の前記置換がＱ２８３Ｅ、Ａ１８０Ｅ、Ｉ２４４Ｔ及びＴ４８Ｅからなる群から選択され、サーモリシン変異体のアミノ酸の位置は、上記特定の配列からなるアミノ酸配列で示されるサーモリシンのアミノ酸配列と対応させて番号付けされている、サーモリシン酵素変異体又はその活性断片、前記サーモリシン酵素変異体を少なくとも１種含む、クリーニング組成物を提供する。【選択図】なし