METHODS FOR PRODUCING NUCLEIC ACID MOLECULES

To provide simple production methods for single-stranded RNA.SOLUTION: Disclosed is a method for producing a single-stranded RNA comprising a step of reacting first and second single-stranded RNAs each having a phosphate group at the 5' end and a third single-stranded RNA having a hydroxy group...

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Bibliographic Details
Main Author SAKATA AKIHIRO
Format Patent
LanguageEnglish
Japanese
Published 01.04.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:To provide simple production methods for single-stranded RNA.SOLUTION: Disclosed is a method for producing a single-stranded RNA comprising a step of reacting first and second single-stranded RNAs each having a phosphate group at the 5' end and a third single-stranded RNA having a hydroxy group at the 5' end with an RNA ligase having a nick repairing activity classified as EC6.5.1.3 according to the IUBMB nomenclature and classification of enzymes to ligate the first, second and third single stranded RNAs.SELECTED DRAWING: None 【課題】一本鎖ＲＮＡの簡便な製造方法を提供すること。【解決手段】５'末端にリン酸基を有する第１の一本鎖ＲＮＡ及び第２の一本鎖ＲＮＡと５'末端に水酸基を有する第３の一本鎖ＲＮＡに、国際生化学・分子生物学連合が酵素番号として定めるＥＣ６．５．１．３に分類され、ニック修復活性を有するＲＮＡリガーゼを作用させ、前記第１の一本鎖ＲＮＡと、前記第２の一本鎖ＲＮＡと、前記第３の一本鎖ＲＮＡとを連結する工程を含む一本鎖ＲＮＡの製造方法。【選択図】なし
Bibliography:Application Number: JP20200214230