METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION THROUGH SINGLE-STEP MULTIPLE TARGETING

To provide methods and compositions for a targeted genetic modification through single-step multiple targeting.SOLUTION: Methods and compositions are provided for modifying a target genomic locus within a cell via a targeting system that utilizes two or more targeting vectors that are capable of rec...

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Bibliographic Details
Main Authors BRIAN ZAMBROWICZ, KEBBEDE ANTENEH, VERA VORONINA, LYNN MACDONALD
Format Patent
LanguageEnglish
Japanese
Published 25.03.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:To provide methods and compositions for a targeted genetic modification through single-step multiple targeting.SOLUTION: Methods and compositions are provided for modifying a target genomic locus within a cell via a targeting system that utilizes two or more targeting vectors that are capable of recombining with one another to form a single contiguous nucleic acid segment. Optionally, the targeting vectors are large targeting vectors (LTVECs). Optionally, the LTVECs are each at least 10 kb in size.SELECTED DRAWING: Figure 1 【課題】単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物を提供する。【解決手段】単一の隣接する核酸セグメントを形成するために互いと組み換えることが可能である２つ以上の標的化ベクターを利用する標的化系を介して、細胞内の標的ゲノム遺伝子座を修飾するための方法及び組成物が提供される。任意に、標的化ベクターは、大型標的化ベクター（ＬＴＶＥＣ）である。任意に、ＬＴＶＥＣはそれぞれ、サイズが少なくとも１０ｋｂである。【選択図】図１
Bibliography:Application Number: JP20200219029