METHOD FOR DETECTING GENE RECOMBINANT

• Main Authors: TANAKA TOMOKI, TAKIYA TOSHIYUKI, KITSUTA KAZUMI, FUKUTOME SHINICHI, SATO EMI, NOMA SATOSHI, MANO JUNICHI, TAKABATAKE REONA, OKUSU HIDEKI, KIKUCHI YOSUKE
• Format: Patent
• Language: English; Japanese
• Published: 18.03.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

To provide a method for detecting a gene recombinant.SOLUTION: A method for detecting a gene recombinant includes: amplifying at least a part of a nucleic acid sequence included in a sample that may contain at least one kind of the gene recombinant by PCR while using a primer which specifically amplifies a recombinant gene derived from the gene recombinant and a primer which specifically amplifies an endogenous gene that a species corresponding to the gene recombinant commonly has and is constituted such that the amplified length of an amplification product of the endogenous gene becomes 95% or less with respect to the amplified length of the amplification product of the recombinant gene; and determining whether or not the abundance ratio of the gene recombinant in the sample is higher than a reference value on the basis of a result of PCR.SELECTED DRAWING: None 【課題】遺伝子組換え体の検出方法を提供する。【解決手段】 遺伝子組換え体の検出方法であって、少なくとも１種の遺伝子組換え体を含む可能性のある試料に含まれる核酸配列の少なくとも一部を、遺伝子組換え体に由来する組換え遺伝子を特異的に増幅するプライマーと、遺伝子組換え体に対応する生物種が共通に有する内在性遺伝子を特異的に増幅し、かつ内在性遺伝子の増幅産物の増幅長が組換え遺伝子の増幅産物の増幅長に対して９５％以下となるよう構成されたプライマーと、を使用して、ＰＣＲにより増幅することと、ＰＣＲの結果に基づいて、試料中の遺伝子組換え体の存在比が基準値より高いか判定することと、を含む、方法。【選択図】 なし