CHIMERIC ANTIGEN RECEPTORS TARGETING EPIDERMAL GROWTH FACTOR RECEPTOR VARIANT III

• Main Authors: BARBRA JOHNSON SASU, WONG OI KWAN, JOYCE CHING CHOU, JULIANNE SMITH, MATHILDE BRUNNHILDE DUSSEAUX
• Format: Patent
• Language: English; Japanese
• Published: 11.03.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

To provide: chimeric antigen receptors (CARs); immune cells (e.g., CAR-T cells) comprising CARs that specifically bind to Epidermal Growth Factor Receptor Variant III (EGFRvIII) (e.g., human EGFRvIII); polynucleotides encoding these CARs; compositions comprising these CAR-T cells; and methods of making and using these CARs and CAR-T cells.SOLUTION: An EGFRvIII-specific CAR comprises an EGFRvIII-binding domain, a transmembrane domain, and an intracellular signaling domain, where the EGFRvIII-binding domain has a specific sequence.SELECTED DRAWING: None 【課題】キメラ抗原受容体（ＣＡＲ）および上皮成長因子受容体変異体ＩＩＩ（ＥＧＦＲｖＩＩＩ）（例えば、ヒトＥＧＦＲｖＩＩＩ）に特異的に結合するＣＡＲを含む免疫細胞（例えば、ＣＡＲ−Ｔ細胞）、これらのＣＡＲをコードするポリヌクレオチド、これらのＣＡＲ−Ｔ細胞を含む組成物およびこれらのＣＡＲおよびＣＡＲ−Ｔ細胞の作製及びその使用方法を提供する。【解決手段】ＥＧＦＲｖＩＩＩ結合ドメイン、膜貫通ドメインおよび細胞内シグナル伝達ドメインを含むＥＧＦＲｖＩＩＩ特異的ＣＡＲであって、ＥＧＦＲｖＩＩＩ結合ドメインが特定の配列を有するＥＧＦＲｖＩＩＩ特異的ＣＡＲ。【選択図】なし