PARTICLES, AFFINITY PARTICLES, REAGENT CONTAINING THE SAME, KIT, AND DETECTION METHOD

• Main Authors: NATORI MAKOTO, NAKAHAMA KAZUMICHI, YAMAUCHI FUMIO, SUDA SAKAE, KANEZAKI KENGO, TSUBAKI KEIICHIRO, SUZUKI MASARO
• Format: Patent
• Language: English; Japanese
• Published: 01.03.2021
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MEASURING; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PHYSICS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP

To provide: particles having little nonspecific adsorption, having reactive functional groups for chemically binding ligands, and being suitable for a coagulation method; particles for coagulation method, having ligands chemically bonded; a reagent for in vivo diagnosis containing the same; a kit; and a detection method of a target substance.SOLUTION: Provided are particles containing a polymer having a unit having a side chain A and a unit having a side chain B. The polymer has a carboxyl group at a terminal of the side chain A, has an ester bond and an amide bond in a molecular structure of the side chain A, and has an alkyl group at a terminal of the side chain B. When dispersed in water, the particles have a Zeta potential of -40 mV to -5 mV.SELECTED DRAWING: None 【課題】非特異吸着が小さく、且つ、リガンドを化学結合するための反応性官能基を有し、凝集法に好適な粒子、リガンドが化学結合してなる凝集法用粒子、及び、これを含む体外診断用試薬、キット、並びに標的物質の検出方法を提供する。【解決手段】側鎖Ａを有するユニットと側鎖Ｂを有するユニットを有するポリマーを含む粒子であって、前記側鎖Ａの末端にカルボキシ基を有し、前記側鎖Ａの分子構造中にエステル結合とアミド結合を有し、前記側鎖Ｂの末端にアルキル基を有し、水中に分散させたときのゼータ電位がマイナス４０ｍＶ以上マイナス５ｍＶ以下であることを特徴とする粒子。【選択図】なし