DETECTION METHOD AND DETECTION DEVICE

• Main Authors: KAWAMURA TATSURO, YANAGAWA HIROTO
• Format: Patent
• Language: English; Japanese
• Published: 03.12.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

To provide a detection method and the like that can achieve improvement of the sensitivity in detecting a target substance by using the surface enhanced fluorescence method.SOLUTION: A detection method includes: coupling a first substance fixed to a metal particle having magnetism and a second substance labeled with a phosphor to a target substance to form a composite (S101); causing the phosphor to emit fluorescence, and irradiating a predetermined area with excitation light having a predetermined wavelength and causing the metal particle to generate localized surface plasmon resonance (S102); applying magnetic field gradient to cause the composite to enter the predetermined area and/or to leave from the predetermined area (S103, S105); detecting over time the fluorescence from the predetermined area (S104, S106); and detecting the target substance based on the amount of change in intensity of the detected fluorescence (S107). The fluorescence emitted by the phosphor is enhanced by the localized surface plasmon resonance generated by the metal particle.SELECTED DRAWING: Figure 6 【課題】表面増強蛍光法を用いて標的物質の検出感度の向上を実現できる検出方法等を提供する。【解決手段】磁性を有する金属粒子に固定された第１物質及び蛍光体で標識された第２物質を標的物質と結合することにより複合体を形成し（Ｓ１０１）、蛍光体に蛍光を放射させ、かつ、金属粒子に局在化表面プラズモン共鳴を生じさせる所定の波長を有する励起光を所定領域に照射し（Ｓ１０２）、磁場勾配を印加して、複合体を所定領域に侵入及び／又は所定領域から離脱させ（Ｓ１０３、Ｓ１０５）、所定領域からの蛍光を経時的に検出し（Ｓ１０４、Ｓ１０６）、検出された蛍光の強度の変化量に基づいて標的物質を検出し（Ｓ１０７）、所定領域において、蛍光体が放射する蛍光は、金属粒子が生じる局在化表面プラズモン共鳴によって増強される。【選択図】図６