MASS ANALYSIS LABELING COMPOSITION, METABOLITE QUANTITATIVE ANALYSIS METHO, AND METABOLITE KINETIC ANALYSIS METHOD

• Main Authors: NAKATANI KOTA, HATA KOSUKE, SOMA YUKI, TAKAHASHI MASATOMO, WAKIMURA AKARI, BABA TAKESHI, TERAUCHI TSUTOMU, YOKOYAMA JUN, IZUMI YOSHIHIRO
• Format: Patent
• Language: English; Japanese
• Published: 19.11.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

To provide a mass analysis labeling composition, metabolite quantitative analysis method, and metabolite kinetic analysis method that can discriminate and evaluate a plurality of labeling compositions to be used in an analysis even when the plurality of labeling compositions undergo a metabolic conversion in a sample.SOLUTION: A mass analysis labeling composition includes labeling compounds of two or more kinds having a metabolite in vivo labelled, in which the labeling compounds of the two or more kinds are labeling compounds of two or more kinds having phosphate compounds of two or more kinds in a series of routes of a metabolic conversion labelled by a stable isotope of one or more kinds including 18O, and the labeling compounds of the two or more kinds are labelled so as to allow transition of a multiple reaction monitoring to mutually distinguish the labeling compounds of two or more kinds from a labeling compound generating by a metabolic conversion of the labeling compound of the two or more kinds. Further, the present disclosure relates to a metabolite quantitative analysis and kinetic analysis method that use the mass analysis labeling composition.SELECTED DRAWING: None 【課題】分析に用いる複数の標識化合物が試料内で代謝変換されたとしても、それらを区別して評価できる質量分析用標識組成物、代謝物の定量分析法、及び代謝物の動態解析法を提供する。【解決手段】生体内の代謝物が標識された２種以上の標識化合物を含む質量分析用標識組成物であって、前記の２種以上の標識化合物は、代謝変換の一連の経路の中の２種以上のリン酸化合物が、１８Ｏを含む１種以上の安定同位体により標識された２種以上の標識化合物であり、前記の２種以上の標識化合物は、前記の２種以上の標識化合物と、前記の２種以上の標識化合物の代謝変換によって生じる標識化合物とが、多重反応モニタリングのトランジションで互いに区別できるように標識されている、質量分析用標識組成物。また、前記質量分析用標識組成物を用いた代謝物の定量分析法及び動態解析法。【選択図】なし