METHODS FOR PROVIDING CHIMERIC ANTIGEN RECEPTOR (CAR) TO INDIVIDUALIZED CELLS

• Main Authors: DOMINIK ECKARDT, OLAF HARDT, ANDREAS BOSIO, HERBEL CHRISTOPH
• Format: Patent
• Language: English; Japanese
• Published: 24.09.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

To provide methods for selecting cancer cell-specific antigens that are not expressed in healthy tissue, in order to design cells that kill/lyse cancer cells without attacking non-tumor cells.SOLUTION: Provided is a method for providing cells containing a chimeric antigen receptor (CAR) specific for one or more target antigens exposed to tumor cells, characterized by providing cell samples containing tumor cells and non-tumor cells and by repeating the following steps: contacting a cell tissue with a conjugate containing a fluorescent moiety and an antigen-recognizing moiety (hereinafter referred to as "conjugate"); removing the conjugate that is not bound from the cell tissue and detecting the cells bound to the conjugate by the fluorescent radiation emitted by the fluorescent moiety of the original conjugate; and extinguishing the fluorescent radiation emitted by the fluorescent moiety of the conjugate.SELECTED DRAWING: None 【課題】非腫瘍細胞を攻撃することなく癌細胞を死滅させる／溶解する細胞を設計するために、健康な組織では発現しない癌細胞に特異的な抗原を選択する方法を提供すること【解決手段】腫瘍細胞及び非腫瘍細胞を含む細胞試料を提供すること、並びに細胞組織と、蛍光部分及び抗原認識部分を含む接合体（以下「接合体」と呼ぶ）とを接触させるステップ、細胞組織から結合していない接合体を取り出し、そして最初の接合体の蛍光部分により発光した蛍光放射線により接合体に結合した細胞を検出するステップ、接合体の蛍光部分により発光した蛍光放射線を消すステップを繰り返すことにより特徴付けられる、腫瘍細胞で曝された１つ以上の標的抗原に特異的なキメラ抗原レセプター（ＣＡＲ）を含む細胞を提供するための方法【選択図】なし