METHODS FOR GENE EXPRESSION REGULATION USING CAS9 PROTEIN EXPRESSED BY TWO VECTORS
To provide a system for expressing Cas9 protein using viral vectors to overcome the limited packaging of one viral vector.SOLUTION: Disclosed is a method for regulating gene expression in vitro comprising a step of introducing into a cell a recombinant vector that expresses a first domain including...
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Main Authors | , |
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Format | Patent |
Language | English Japanese |
Published |
10.09.2020
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Subjects | |
Online Access | Get full text |
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Summary: | To provide a system for expressing Cas9 protein using viral vectors to overcome the limited packaging of one viral vector.SOLUTION: Disclosed is a method for regulating gene expression in vitro comprising a step of introducing into a cell a recombinant vector that expresses a first domain including the N-terminal of Cas9 protein and a recombinant vector that expresses a second domain including the C-terminal of Cas9 protein, where the first and second domains fuse to form a full-length Cas9 protein. Also disclosed are: a composition comprising the recombinant vectors; a kit for regulating gene expression and a method for intracellular production of Cas9. A transformed cell introduced with a virus vector packaging the first domain and another virus vector packaging the second domain, and a composition comprising the virus produced therefrom are also disclosed.SELECTED DRAWING: Figure 1
【課題】ウイルスベクターのパッケージングの限界を克服して、ウイルスベクターを利用してCas9タンパク質を発現させることができるシステムの提供。【解決手段】Cas9タンパク質のN−末端を含む第一のドメインを発現する組換えベクター及びC−末端を含む第二のドメインを発現する組換えベクターを各々細胞内に導入する工程を含む、インビトロで遺伝子発現を調節する方法であって、前記第一のドメイン及び第二のドメインが融合し、完全なCas9タンパク質を形成する、方法。前記組換えベクターを含む組成物、遺伝子発現調節用キット、及びCas9タンパク質の細胞内製造方法。また、前記第一のドメインをパッケージングするウイルスベクター及び第二のドメインをパッケージングするウイルスベクターが導入された形質転換細胞及びこれらから産生されたウイルスを含む組成物。【選択図】図1 |
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Bibliography: | Application Number: JP20200081896 |