PARTICLE MANIPULATION DEVICE

To provide a manipulation tube that enables accurate evaluation of a recovery ratio of biological components such as nucleic acids recovered in an eluate, and a device including the manipulation tube.SOLUTION: Provided is a manipulation tube 1 for manipulating a target component, comprising: a hollo...

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Main Authors YOMO MASAMITSU, MURAMATSU AKIRA, KANAI MASAKI, JIKUYA HIROYUKI, YAMANO AYAKA, FUJIWARA SEIYA, OHASHI TETSUO
Format Patent
LanguageEnglish
Japanese
Published 10.09.2020
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:To provide a manipulation tube that enables accurate evaluation of a recovery ratio of biological components such as nucleic acids recovered in an eluate, and a device including the manipulation tube.SOLUTION: Provided is a manipulation tube 1 for manipulating a target component, comprising: a hollow tube having an open end 5 on one side and a closed end on the other side, and having a manipulation tube portion a and a recovery tube portion b; a manipulation medium 3 filled in the manipulation tube portion a such that a gel layer 3g and an aqueous liquid layer 3l are alternately layered, and the layer lengths of the gel layer and the layer length of the aqueous liquid layer each being defined by the lengthwise length of the hollow tube; a recovery medium 4 filled in the recovery tube portion b such that the gel layer and the aqueous liquid layer contacting the closed end are alternately layered, and the aqueous liquid layer contacting the closed end having a predetermined volume and the layer length of the gel layer being defined by the lengthwise length of the hollow tube; and magnetic material particles 6 for capturing and carrying the target component.SELECTED DRAWING: Figure 1 【課題】溶出液中に回収される核酸などの生体成分の正確な回収率評価を可能にする操作管及び該操作管を備えるデバイスを提供する。【解決手段】対象成分を操作するための操作管１であって、一方に開口端５及び他方に閉口端を有する中空管であって、操作用管部ａと回収用管部ｂとを有する中空管；前記操作用管部ａ内において、ゲル層３ｇ及び水系液体層３ｌが交互に重層するように充填された操作用媒体３であって、前記ゲル層及び前記水系液体層のそれぞれの層長が前記中空管の長手方向の長さで定められる操作用媒体；前記回収用管部ｂ内において、ゲル層及び前記閉口端に接する水系液体層が重層するように充填された回収用媒体４であって、前記閉口端に接する前記水系液体層が所定の体積を有し、かつ前記ゲル層の層長が前記中空管の長手方向の長さで定められる回収用媒体；及び、対象成分を捕捉し、運搬する磁性体粒子６；を備えるように構成された操作管。【選択図】図１
Bibliography:Application Number: JP20190043228