METHOD AND REAGENT KIT FOR MEASURING CHOLESTEROL INCORPORATION EFFICIENCY OF LIPOPROTEIN

To provide a method for measuring the cholesterol incorporation efficiency of lipoprotein.SOLUTION: The method includes the steps of: bringing lipoprotein in a sample obtained from a subject, tag-added cholesterol, and a labeled capture body which binds specifically to a tag into each other to form...

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Bibliographic Details
Main Authors YOSHIKAWA KEIKO, MIWA KEIKO, MURAKAMI KATSUHIRO, KUBO TAKUYA, HARADA SHU, KIRIYAMA MARIA
Format Patent
LanguageEnglish
Japanese
Published 20.08.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:To provide a method for measuring the cholesterol incorporation efficiency of lipoprotein.SOLUTION: The method includes the steps of: bringing lipoprotein in a sample obtained from a subject, tag-added cholesterol, and a labeled capture body which binds specifically to a tag into each other to form a complex including the lipoprotein, the tag-added cholesterol, and the labeled capture body; and detecting a signal generated from the label in the complex, the tag-added cholesterol being the base of lecitin-cholesterol acyl transferase.SELECTED DRAWING: None 【課題】リポタンパク質のコレステロール取り込み能を測定する方法を提供する。【解決手段】被検者から得られた試料中のリポタンパク質と、タグ付加コレステロールと、タグに特異的に結合する標識捕捉体とを接触させることにより、リポタンパク質とタグ付加コレステロールと前記標識捕捉体とを含む複合体を形成する工程と、複合体に含まれる標識により生じるシグナルを検出する工程とを含み、タグ付加コレステロールが、レシチン-コレステロールアシルトランスフェラーゼの基質となる、コレステロールを測定する工程を含む。【選択図】なし
Bibliography:Application Number: JP20200047833