ENHANCED UMBILICAL CORD-DERIVED ADHERENT STEM CELL, METHOD FOR PRODUCING THE SAME, AND APPLICATION OF THE SAME

• Main Authors: KANG AHREUM, KIM HYE SUN, YU JI MIN, KIM JI HYE, SHIN JEONG MIN
• Format: Patent
• Language: English; Japanese
• Published: 14.05.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

To provide an enhanced umbilical cord-derived adherent stem cell, a method for producing the same, and application of the same.SOLUTION: There is provided an umbilical cord-derived adherent stem cell having one or more characteristics selected from the following (a) to (e): a) expressing one or more selected from the group consisting of COL1A1, IGFBP4, TAGLN, STC1, LRRC17 and IL33 further more compared with a bone marrow stem cell; b) expressing one or more selected from the group consisting of CCND1, SERPINE1, PRNP and CYP1B1 further less compared with the bone marrow stem cell; c) maintaining the mode of an adherent fibroblast while performing subculture; d) differentiation potency to adipocyte, osteocyte or chondrocyte; and e) one or more surface antigen characteristics selected from the group consisting of CD200+, Tra-1-60-, CD3-, CD1a-, CD11c-, CD16-, CD86-, CD8a-, CD40-, CD141+, CD61+, CD87+, MICA/B- and SSEA4+.SELECTED DRAWING: Figure 4A 【課題】向上された臍帯由来付着型幹細胞、その製造方法及びその用途の提供。【解決手段】下記（ａ）ないし（ｅ）から選択される１以上の特性を有する臍帯由来付着型幹細胞。ａ）ＣＯＬ１Ａ１、ＩＧＦＢＰ４、ＴＡＧＬＮ、ＳＴＣ１、ＬＲＲＣ１７及びＩＬ３３からなる群から選択される１以上が、骨髄幹細胞に比べ、さらに多く発現されること、ｂ）ＣＣＮＤ１、ＳＥＲＰＩＮＥ１、ＰＲＮＰ及びＣＹＰ１Ｂ１からなる群から選択される１以上が、骨髄幹細胞に比べ、さらに少なく発現されること、ｃ）継代培養する間、付着型線維芽細胞の形態を維持すること、ｄ）脂肪細胞、骨細胞または軟骨細胞への分化能；及びｅ）ＣＤ２００＋、Ｔｒａ−１−６０−、ＣＤ３−、ＣＤ１ａ−、ＣＤ１１ｃ−、ＣＤ１６−、ＣＤ８６−、ＣＤ８ａ−、ＣＤ４０−、ＣＤ１４１＋、ＣＤ６１＋、ＣＤ８７＋、ＭＩＣＡ／Ｂ−及びＳＳＥＡ４＋からなる群から選択される１以上の表面抗原特性。【選択図】図４Ａ