ARTICLE AND METHOD FOR DETECTING AEROBIC BACTERIA

• Main Authors: SAILAJA CHANDRAPATI, TERA M NORDBY
• Format: Patent
• Language: English; Japanese
• Published: 14.05.2020
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

To provide a device capable of promptly detecting and counting aerobic bacteria.SOLUTION: A device (200) for culturing and detecting microorganisms, comprises: a substrate sheet (10) having a first major surface (10a) and a second major surface (10b); a cover sheet (20) attached to the substrate sheet; a sample-receiving zone disposed between the substrate sheet and the cover sheet; a first layer (12) adhered to the first major surface of the substrate sheet and comprising a substantially dry, cold-water-soluble first hydrogel-forming composition; an air-permeable membrane (32) adhered to the substrate sheet; a plurality of indicator agents for detecting enzyme activities of glycosidase, alkyl esterase, and phosphatase; and a redox indicator; the indicator agents being disposed in the substrate sheet in fluid communication with the sample-receiving zone or at least one layer adhered to the cover sheet when a predetermined volume of aqueous liquid is deposited in the sample-receiving zone.SELECTED DRAWING: Figure 5 【課題】好気性菌を迅速に検出及び計数することができる装置の提供。【解決手段】第１の主表面（１０ａ）及び第２の主表面（１０ｂ）を有する基材シート（１０）と、前記基材シートに取り付けられたカバーシート（２０）と、前記基材シートとカバーシートとの間に配置されたサンプル受領領域と、前記基材シートの第１の主表面に接着された、実質的に乾燥しており、冷水溶解性の第１のヒドロゲル形成組成物を含む第１の層（１２）と、前記基材シートに接着された通気性膜（３２）と、グリコシダーゼ、アルキルエステラーゼ及びホスファターゼのそれぞれの酵素活性を検出するための複数の指示薬と酸化還元指示薬とが、所定量の水性液体が前記サンプル受領領域に配置されたときにサンプル受領領域と流体連通する前記基材シート又はカバーシートに接着された少なくとも１つの層上に配置されている、微生物を培養及び検出するための装置（２００）。【選択図】図５