ALKALINE PHOSPHATASES WITH NO ACTIVITY

• Main Author: MATSUBA TAKAO
• Format: Patent
• Language: English; Japanese
• Published: 26.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

To provide alkaline phosphatases with no activity usable for preventing non-specific adsorption of genetically engineered alkaline phosphatases produced in animal cells.SOLUTION: Disclosed herein is an alkaline phosphatase having no or reduced activity as compared with a wild type alkaline phosphatase, in whose amino acid sequence, serine at position 92 is deleted or substituted with another amino acid. In an embodiment of the alkaline phosphatase with no activity, another amino acid is aspartic acid or isoleucine.SELECTED DRAWING: None 【課題】遺伝子組換え技術を用いて動物細胞で作製したアルカリホスファターゼの非特異的吸着を防止するために利用できる、無活性アルカリホスファターゼを提供すること。【解決手段】アルカリホスファターゼのアミノ酸配列の９２番目のアミノ酸であるセリンを欠失させるか、別のアミノ酸に置換したアミノ酸配列を含み、野生型のアルカリホスファターゼと比較して活性が低下した無活性アルカリホスファターゼ。別のアミノ酸が、アスパラギン酸またはイソロイシンである、無活性アルカリホスファターゼ。【選択図】なし