MICROSCOPE APPARATUS

• Main Authors: KANEKO YASUTOSHI, SASE ICHIRO, FUKUI TATSUO
• Format: Patent
• Language: English; Japanese
• Published: 19.03.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS; TESTING

To provide a microscope apparatus for improving quality of a reconstructed image using imaging result captured at a plurality of imaging positions.SOLUTION: A microscope apparatus 1 includes: an illumination optical system 4 that radiates activation light L2 to activate a part of fluorescent material included in a sample W and excitation light L1 to excite at least a part of the activated fluorescent material; an image forming optical system 5 that has an objective lens 21 and an astigmatic optical system 61 that generates astigmatism to at least a part of fluorescence from the fluorescent material; and forms an image of the fluorescence; an image-capturing unit 6 that captures an image formed by the image forming optical system 5; a drive unit 50 that moves an image-capturing position in the sample W along an optical axis-direction 21a of the objective lens 21; and a control unit 42, where the control unit 42 causes the image-capturing unit 50 to capture images in a plurality of numbers of frames respectively at a first image-capturing position and at a second image-capturing position different from the first image-capturing position.SELECTED DRAWING: Figure 1 【課題】複数の撮像位置で撮像した撮像結果を用いた再構成画像の品質を向上する顕微鏡装置を提供する。【解決手段】試料Ｗに含まれる蛍光物質の一部を活性化する活性化光Ｌ２と、活性化された蛍光物質の少なくとも一部を励起する励起光Ｌ１を照射する照明光学系４と、対物レンズ２１と、蛍光物質からの蛍光の少なくとも一部に対して非点収差を発生させる非点収差光学系６１を有し、蛍光の像を形成する結像光学系５と、結像光学系５が形成した像を撮像する撮像部６と、試料Ｗにおける撮像位置を対物レンズ２１の光軸方向２１ａに沿って移動する駆動部５０と、制御部４２とを有する顕微鏡１であって、制御部４２は、撮像部５０に、第１の撮像位置および第１の撮像位置とは異なる第２の撮像位置において、それぞれ複数のフレーム数で撮像させる。【選択図】図１